Prostaglandin E₂ glyceryl ester is an endogenous agonist of the nucleotide receptor P2Y₆.

ABSTRACT: Cyclooxygenase-2 catalyses the biosynthesis of prostaglandins from arachidonic acid but also the biosynthesis of prostaglandin glycerol esters (PG-Gs) from 2-arachidonoylglycerol. Previous studies identified PG-Gs as signalling molecules involved in inflammation. Thus, the glyceryl ester of prostaglandin E₂, PGE₂-G, mobilizes Ca²⁺ and activates protein kinase C and ERK, suggesting the involvement of a G protein-coupled receptor (GPCR). To identify the endogenous receptor for PGE₂-G, we performed a subtractive screening approach where mRNA from PGE₂-G response-positive and -negative cell lines was subjected to transcriptome-wide RNA sequencing analysis. We found several GPCRs that are only expressed in the PGE₂-G responder ce

SUBMITTER: Bruser A 

PROVIDER: S-EPMC5443783 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature