Genomewide Mutational Diversity in <i>Escherichia coli</i> Population Evolving in Prolonged Stationary Phase.

ABSTRACT: Prolonged stationary phase is an approximation of natural environments presenting a range of stresses. Survival in prolonged stationary phase requires alternative metabolic pathways for survival. This study describes the repertoire of mutations accumulating in starving Escherichia coli populations in lysogeny broth. A wide range of mutations accumulates over the course of 1 month in stationary phase. Single nucleotide polymorphisms (SNPs) constitute 64% of all mutations. A majority of these mutations are nonsynonymous and are located at conserved loci. There is an increase in genetic diversity in the evolving populations over time. Computer simulations of evolution in stationary phase suggest that the maximum frequency of mutations observed in our experimental populations cannot be

SUBMITTER: Chib S 

PROVIDER: S-EPMC5444009 | biostudies-literature | 2017 May-Jun

REPOSITORIES: biostudies-literature