Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice.

ABSTRACT:

Background

Advances in optical imaging modalities and the continued evolution of genetically-encoded fluorescent proteins are coming together to facilitate the study of cell behavior at high resolution in living organisms. As a result, imaging using autofluorescent protein reporters is gaining popularity in mouse transgenic and targeted mutagenesis applications.

Results

We have used embryonic stem cell-mediated transgenesis to label cells at sub-cellular resolution in vivo, and to evaluate fusion of a human histone protein to green fluorescent protein for ubiquitous fluorescent labeling of nucleosomes in mice. To this end we have generated embryonic stem cells and a corresponding strain of mice that is viable and fertile and exhibits widespread chromatin-localized reporter e

SUBMITTER: Hadjantonakis AK 

PROVIDER: S-EPMC544401 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature