Hepatic gene therapy rescues high-fat diet responses in circadian Clock mutant mice.

ABSTRACT:

Objective

Circadian Clock gene mutant mice show dampened 24-h feeding rhythms and an increased sensitivity to high-fat diet (HFD) feeding. Restricting HFD access to the dark phase counteracts its obesogenic effect in wild-type mice. The extent to which altered feeding rhythms are causative for the obesogenic phenotype of Clock mutant mice, however, remains unknown.

Methods

Metabolic parameters of wild-type (WT) and Clock^Δ19 mutant mice (MT) were investigated under ad libitum and nighttime restricted HFD feeding. Liver circadian clock function was partially rescued by hydrodynamic tail vein delivery of WT-Clock DNA vectors in mutant mice and transcriptional, metabolic, endocrine and behavioral rhythms studied.

Results

SUBMITTER: Meyer-Kovac J 

PROVIDER: S-EPMC5444075 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature