A Simple Marker-Assisted 3D Nanometer Drift Correction Method for Superresolution Microscopy.

ABSTRACT: High-precision fluorescence microscopy such as superresolution imaging or single-particle tracking often requires an online drift correction method to maintain the stability of the three-dimensional (3D) position of the sample at a nanometer precision throughout the entire data acquisition process. Current online drift correction methods require modification of the existing two-dimensional (2D) fluorescence microscope with additional optics and detectors, which can be cumbersome and limit its use in many biological laboratories. Here we report a simple marker-assisted online drift correction method in which all 3D positions can be derived from fiducial markers on the coverslip of the sample on a standard 2D fluorescence microscope without additional optical components. We validate this met

SUBMITTER: Ma H 

PROVIDER: S-EPMC5444076 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature