Effect of Ca²⁺ to Sphingomyelin Investigated by Sum Frequency Generation Vibrational Spectroscopy.

ABSTRACT: The interactions between Ca²⁺ ions and sphingomyelin play crucial roles in a wide range of cellular activities. However, little is known about the molecular details of the interactions at interfaces. In this work, we investigated the interactions between Ca²⁺ ions and egg sphingomyelin (ESM) Langmuir monolayers at the air/water interface by subwavenumber high-resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS). We show that Ca²⁺ ions can induce ordering of the acyl chains in the ESM monolayer. An analysis of the one alkyl-chain-deuterated ESM revealed that the Ca²⁺ ions do not affect the N-linked saturated fatty acid chain, although they make the sphingosine backbone become ordered. Further analysis of the SFG-VS

SUBMITTER: Feng RJ 

PROVIDER: S-EPMC5444077 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature