Type 2 diabetes and obesity induce similar transcriptional reprogramming in human myocytes.

ABSTRACT:

Background

Skeletal muscle is one of the primary tissues involved in the development of type 2 diabetes (T2D). The close association between obesity and T2D makes it difficult to isolate specific effects attributed to the disease alone. Therefore, here we set out to identify and characterize intrinsic properties of myocytes, associated independently with T2D or obesity.

Methods

We generated and analyzed RNA-seq data from primary differentiated myotubes from 24 human subjects, using a factorial design (healthy/T2D and non-obese/obese), to determine the influence of each specific factor on genome-wide transcription. This setup enabled us to identify intrinsic properties, originating from muscle precursor cells and retained in the corresponding myocytes. Bioinformatic and stati

SUBMITTER: Varemo L 

PROVIDER: S-EPMC5444103 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature