Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation.

ABSTRACT: In vivo and ex vivo imaging were used to investigate the function of galectin-3 (Gal-3) during the process of leukocyte recruitment to the inflamed microcirculation. The cremasteric microcirculation of wild-type (C57BL/6), Gal-3^-/-, and CX₃CR1^gfp/+ mice were assessed by intravital microscopy after PBS, IL-1β, TNF-α, or recombinant Gal-3 treatment. These cellular responses were investigated further using flow-chamber assays, confocal microscopy, flow cytometry, PCR analysis, and proteome array. We show that mechanisms mediating leukocyte slow rolling and emigration are impaired in Gal-3^-/- mice, which could be because of impaired expression of cell adhesion molecules and an altered cell surface glycoproteome. Local (intrascrotal) administration of

SUBMITTER: Gittens BR 

PROVIDER: S-EPMC5444525 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature