Elucidation of new condition-dependent roles for fructose-1,6-bisphosphatase linked to cofactor balances.

ABSTRACT: The cofactor balances in metabolism is of paramount importance in the design of a metabolic engineering strategy and understanding the regulation of metabolism in general. ATP, NAD+ and NADP+ balances are central players linking the various fluxes in central metabolism as well as biomass formation. NADP+ is especially important in the metabolic engineering of yeasts for xylose fermentation, since NADPH is required by most yeasts in the initial step of xylose utilisation, including the fast-growing Kluyveromyces marxianus. In this simulation study of yeast metabolism, the complex interplay between these cofactors was investigated; in particular, how they may affect the possible roles of fructose-1,6-bisphosphatase, the pentose phosphate pathway, glycerol production and the pyruvate dehydrog

SUBMITTER: Schabort DTWP 

PROVIDER: S-EPMC5444679 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature