Thermodynamic framework to assess low abundance DNA mutation detection by hybridization.

ABSTRACT: The knowledge of genomic DNA variations in patient samples has a high and increasing value for human diagnostics in its broadest sense. Although many methods and sensors to detect or quantify these variations are available or under development, the number of underlying physico-chemical detection principles is limited. One of these principles is the hybridization of sample target DNA versus nucleic acid probes. We introduce a novel thermodynamics approach and develop a framework to exploit the specific detection capabilities of nucleic acid hybridization, using generic principles applicable to any platform. As a case study, we detect point mutations in the KRAS oncogene on a microarray platform. For the given platform and hybridization conditions, we demonstrate the multiplex detection capa

SUBMITTER: Willems H 

PROVIDER: S-EPMC5444680 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature