Circumvent the uncertainty in the applications of transcriptional signatures to tumor tissues sampled from different tumor sites.

ABSTRACT: The expression measurements of thousands of genes are correlated with the proportions of tumor epithelial cell (PTEC) in clinical samples. Thus, for a tumor diagnostic or prognostic signature based on a summarization of expression levels of the signature genes, the risk score for a patient may dependent on the tumor tissues sampled from different tumor sites with diverse PTEC for the same patient. Here, we proposed that the within-samples relative expression orderings (REOs) based gene pairs signatures should be insensitive to PTEC variations. Firstly, by analysis of paired tumor epithelial cell and stromal cell microdissected samples from 27 cancer patients, we showed that above 80% of gene pairs had consistent REOs between the two cells, indicating these REOs would be independent of PTEC

SUBMITTER: Cheng J 

PROVIDER: S-EPMC5444741 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature