ToNER: A tool for identifying nucleotide enrichment signals in feature-enriched RNA-seq data.

ABSTRACT:

Background

Biochemical methods are available for enriching 5' ends of RNAs in prokaryotes, which are employed in the differential RNA-seq (dRNA-seq) and the more recent Cappable-seq protocols. Computational methods are needed to locate RNA 5' ends from these data by statistical analysis of the enrichment. Although statistical-based analysis methods have been developed for dRNA-seq, they may not be suitable for Cappable-seq data. The more efficient enrichment method employed in Cappable-seq compared with dRNA-seq could affect data distribution and thus algorithm performance.

Results

We present Transformation of Nucleotide Enrichment Ratios (ToNER), a tool for statistical modeling of enrichment from RNA-seq data obtained from enriched and unenriched libraries. The tool calcula

SUBMITTER: Promworn Y 

PROVIDER: S-EPMC5444824 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature