Cellular migration, transition and interaction during regeneration of the sponge Hymeniacidon heliophila.

ABSTRACT: Sponges have a high capacity for regeneration and this process improves biomass production in some species, thus contributing to a solution for the biomass supply problem for biotechnological applications. The aim of this work is to characterize the dynamics of cell behavior during the initial stages of sponge regeneration, using bright-field microscopy, confocal microscopy and SEM. We focused on the first 20 h of regeneration, during which blastema formation and epithelium initialization occur. An innovative sponge organotypic culture of the regenerating internal region is described and investigated by confocal microscopy, cell transplantation and vital staining. Cell-cell interaction and cell density are shown to affect events in morphogenesis such as epithelial/mesenchymal and mesenchym

SUBMITTER: Coutinho CC 

PROVIDER: S-EPMC5444830 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature