A Method for In-Depth Structural Annotation of Human Serum Glycans That Yields Biological Variations.

ABSTRACT: Glycosylation is an important post-translational modification of proteins present in the vast majority of human proteins. For this reason, they are potentially new sources of biomarkers and active targets of therapeutics and vaccines. However, the absence of a biosynthetic template as in the genome and the general complexity of the structures have limited the development of methods for comprehensive structural analysis. Even now, the exact structures of many abundant N-glycans in serum are not known. Structural elucidation of oligosaccharides remains difficult and time-consuming. Here, we introduce a means of rapidly identifying released N-glycan structures using their accurate masses and retention times based on a glycan library. This serum glycan library, significantly expanded from a pr

SUBMITTER: Song T 

PROVIDER: S-EPMC5444872 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature