Deep interactome profiling of membrane proteins by co-interacting protein identification technology.

ABSTRACT: Affinity purification coupled to mass spectrometry (AP-MS) is the method of choice for analyzing protein-protein interactions, but common protocols frequently recover only the most stable interactions and tend to result in low bait yield for membrane proteins. Here, we present a novel, deep interactome sequencing approach called CoPIT (co-interacting protein identification technology), which allows comprehensive identification and analysis of membrane protein interactomes and their dynamics. CoPIT integrates experimental and computational methods for a coimmunoprecipitation (Co-IP)-based workflow from sample preparation for mass spectrometric analysis to visualization of protein-protein interaction networks. The approach particularly improves the results for membrane protein interactomes,

SUBMITTER: Pankow S 

PROVIDER: S-EPMC5444904 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature