Bile duct ligation-induced biliary hyperplasia, hepatic injury, and fibrosis are reduced in mast cell-deficient Kit^W-sh mice.

ABSTRACT: Activated mast cells (MCs) release histamine (HA) and MCs infiltrate the liver following bile duct ligation (BDL), increasing intrahepatic bile duct mass (IBDM) and fibrosis. We evaluated the effects of BDL in MC-deficient (Kit^W-sh ) mice. Wild-type (WT) and Kit^W-sh mice were subjected to sham or BDL for up to 7 days and Kit^W-sh mice were injected with cultured mast cells or 1× phosphate-buffered saline (PBS) before collecting serum, liver, and cholangiocytes. Liver damage was assessed by hematoxylin and eosin and alanine aminotransferase levels. IBDM was detected by cytokeratin-19 expression and proliferation by Ki-67 immunohistochemistry (IHC). Fibrosis was detected by IHC, hydroxyproline content, and by qPCR for fibrotic markers. Hepatic stellate cell (H

SUBMITTER: Hargrove L 

PROVIDER: S-EPMC5444972 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature