NOSH-NBP, a Novel Nitric Oxide and Hydrogen Sulfide- Releasing Hybrid, Attenuates Ischemic Stroke-Induced Neuroinflammatory Injury by Modulating Microglia Polarization.

ABSTRACT: NOSH-NBP, a novel nitric oxide (NO) and hydrogen sulfide (H₂S)-releasing hybrid, protects brain from ischemic stroke. This study mainly aimed to investigate the therapeutic effect of NOSH-NBP on ischemic stroke and the underlying mechanisms. In vivo, transient middle cerebral artery occlusion (tMCAO) was performed in C57BL/6 mice, with NO-NBP and H₂S-NBP as controls. NO and H₂S scavengers, carboxy-PTIO and BSS, respectively, were used to quench NO and H₂S of NOSH-NBP. In vitro, BV₂ microglia/BMDM were induced to the M1/2 phenotype, and conditioned medium (CM) experiments in BV₂ microglia, neurons and b.End3 cerebral microvascular endothelial cells (ECs) were performed. Microglial/macrophage activation/polarizat

SUBMITTER: Ji J 

PROVIDER: S-EPMC5445131 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature