Sources of variability in the measurement of Ascaris lumbricoides infection intensity by Kato-Katz and qPCR.

ABSTRACT:

Background

Understanding and quantifying the sources and implications of error in the measurement of helminth egg intensity using Kato-Katz (KK) and the newly emerging "gold standard" quantitative polymerase chain reaction (qPCR) technique is necessary for the appropriate design of epidemiological studies, including impact assessments for deworming programs.

Methods

Repeated measurements of Ascaris lumbricoides infection intensity were made from samples collected in western Kenya using the qPCR and KK techniques. These data were combined with data on post-treatment worm expulsions. Random effects regression models were used to quantify the variability associated with different technical and biological factors for qPCR and KK diagnosis. The relative precision of these methods

SUBMITTER: Easton AV 

PROVIDER: S-EPMC5445470 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature