QuantGenius: implementation of a decision support system for qPCR-based gene quantification.

ABSTRACT:

Background

Quantitative molecular biology remains a challenge for researchers due to inconsistent approaches for control of errors in the final results. Due to several factors that can influence the final result, quantitative analysis and interpretation of qPCR data are still not trivial. Together with the development of high-throughput qPCR platforms, there is a need for a tool allowing for robust, reliable and fast nucleic acid quantification.

Results

We have developed "quantGenius" ( http://quantgenius.nib.si ), an open-access web application for a reliable qPCR-based quantification of nucleic acids. The quantGenius workflow interactively guides the user through data import, quality control (QC) and calculation steps. The input is machine- and chemistry-independent. Quant

SUBMITTER: Baebler S 

PROVIDER: S-EPMC5445471 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature