Compaction of Duplex Nucleic Acids upon Native Electrospray Mass Spectrometry.

ABSTRACT: We report on the fate of nucleic acids conformation in the gas phase as sampled using native mass spectrometry coupled to ion mobility spectrometry. On the basis of several successful reports for proteins and their complexes, the technique has become popular in structural biology, and the conformation survival becomes more and more taken for granted. Surprisingly, we found that DNA and RNA duplexes, at the electrospray charge states naturally obtained from native solution conditions (≥100 mM aqueous NH₄OAc), are significantly more compact in the gas phase compared to the canonical solution structures. The compaction is observed for all duplex sizes (gas-phase structures are more compact than canonical B-helices by ∼20% for 12-bp, and by up to ∼30% for 36-bp duplexes), and for DN

SUBMITTER: Porrini M 

PROVIDER: S-EPMC5445532 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature