Functionally and morphologically damaged mitochondria observed in auditory cells under senescence-inducing stress.

ABSTRACT: We aimed at determining the mitochondrial function in premature senescence model of auditory cells. Short exposure to H₂O₂ (1 h, 0.1 mM) induced premature cellular senescence in House Ear Institute-Organ of Corti 1 auditory cells. The transmission electron microscopy analysis revealed that damaged mitochondria and autophagosomes containing dense organelles appeared in the auditory cells after short exposure to H₂O₂. The branch and junction parameters of the skeletonized image of the mitochondria were found to decrease significantly in H₂O₂-treated cells. A branched reticulum of tubules was poorly formed, featuring coexistence of numerous tiny clusters along with few relatively large entities in the H₂O₂

SUBMITTER: Kamogashira T 

PROVIDER: S-EPMC5445612 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature