Regulation of cell cycle by the anaphase spindle midzone.

ABSTRACT:

Background

A number of proteins accumulate in the spindle midzone and midbody of dividing animal cells. Besides proteins essential for cytokinesis, there are also components essential for interphase functions, suggesting that the spindle midzone and/or midbody may play a role in regulating the following cell cycle.

Results

We microsurgically severed NRK epithelial cells during anaphase or telophase, such that the spindle midzone/midbody was associated with only one of the daughter cells. Time-lapse recording of cells severed during early anaphase indicated that the cell with midzone underwent cytokinesis-like cortical contractions and progressed normally through the interphase, whereas the cell without midzone showed no cortical contraction and an arrest or substantial delay

SUBMITTER: Murata-Hori M 

PROVIDER: S-EPMC544570 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature