Isolation and characterization of microsatellite markers for Sturnira parvidens and cross-species amplification in Sturnira species.

ABSTRACT:

Background

Sturnira is one of the most species-rich genera in the Neotropics, and it is found from Mexico and the Lesser Antilles to Argentina. This genus forms a well-supported monophyletic clade with at least twenty-one recognized species, as well as several others under taxonomic review. Sturnira parvidens is a widespread frugivorous bat of the deciduous forests of the Neotropics, is highly abundant, and is a major component in fruit dispersal to regenerate ecosystems.

Methods

We used a technique based on Illumina paired-end sequencing of a library highly enriched for microsatellite repeats to develop loci for S. parvidens. We analyzed millions of resulting reads with specialized software to extract those reads that contained di-, tri-, tetra-, penta-

SUBMITTER: Gutierrez EG 

PROVIDER: S-EPMC5445947 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature