Kemp Eliminase Activity of Ketosteroid Isomerase.

ABSTRACT: Kemp eliminases represent the most successful class of computationally designed enzymes, with rate accelerations of up to 10⁹-fold relative to the rate of the same reaction in aqueous solution. Nevertheless, several other systems such as micelles, catalytic antibodies, and cavitands are known to accelerate the Kemp elimination by several orders of magnitude. We found that the naturally occurring enzyme ketosteroid isomerase (KSI) also catalyzes the Kemp elimination. Surprisingly, mutations of D38, the residue that acts as a general base for its natural substrate, produced variants that catalyze the Kemp elimination up to 7000-fold better than wild-type KSI does, and some of these variants accelerate the Kemp elimination more than the computationally designed Kemp eliminases. Ana

SUBMITTER: Lamba V 

PROVIDER: S-EPMC5446047 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature