Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue.

ABSTRACT: The creation of physiologically-relevant human cardiac tissue with defined cell structure and function is essential for a wide variety of therapeutic, diagnostic, and drug screening applications. Here we report a new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) into predefined 3D constructs. At packing densities that approximate native myocardium (10⁸-10⁹ cells/ml), these hiPSC-CM-derived 3D tissues demonstrate significantly improved cell viability, metabolic activity, and intercellular connection when compared to constructs with random cell distribution. Moreover, the patterned hiPSC-CMs within the constructs exhibit significantly greater levels of contractile stress, beat fr

SUBMITTER: Serpooshan V 

PROVIDER: S-EPMC5446052 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature