A versatile cell-penetrating peptide-adaptor system for efficient delivery of molecular cargos to subcellular destinations.

ABSTRACT: Cell penetrating peptides have long held great potential for delivery of biomolecular cargos for research, therapeutic and diagnostic purposes. They allow rapid, relatively nontoxic passage of a wide variety of biomolecules through the plasma membranes of living cells. However, CPP-based research tools and therapeutics have been stymied by poor efficiency in release from endosomes and a great deal of effort has been made to solve this 'endosomal escape problem.' Previously, we showed that use of a reversible, noncovalent coupling between CPP and cargo using calmodulin and a calmodulin binding motif allowed efficient delivery of cargo proteins to the cytoplasm in baby hamster kidney and other mammalian cell lines. The present report demonstrates the efficacy of our CPP-adaptor scheme for ef

SUBMITTER: Ngwa VM 

PROVIDER: S-EPMC5446193 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature