Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal.

ABSTRACT: A novel sandwich-assay electrochemical immunosensor for simultaneous determination of autophagic biomarkers was introduced for the first time, the gold-reduced grapheme oxide nanocomposite (Au/r-GO) set as a good conductive platform with super high specific area, and provided more binding sites for the both antibodies of Beclin-1 and LC3B-II. While Au nanocages (AuNCs) served as good conductive platform to encapsulate a large amount of redox probe and secondary antibodies for signal amplification, due to the abundant reactive oxygen functional groups on its surface. Through differential pulse voltammetry (DPV) measurements, two separate signals can be detected directly in a single run, which represent the existence of Belin-1 and LC3B-II. Under optimized conditions, the electrochemical imm

SUBMITTER: Wang G 

PROVIDER: S-EPMC5446417 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature