CCR6⁺ B lymphocytes responding to tumor cell-derived CCL20 support hepatocellular carcinoma progression via enhancing angiogenesis.

ABSTRACT:

Background & aims

Different immune cells in tumor microenvironment shape tumor progression. CCL20 over-expression was reported as one of the "stemness" trait in TP53 mutated hepatocellular carcinoma (HCC). We aimed to understand the effect of CCL20 on HCC progression.

Methods

In two HCC cohort patients (n=95, n=85 respectively), serum CCL20 concentration was quantified by using ELISA. Expressions of CCL20 and CCR6 in 41 paired HCC tumor and adjacent non-tumor tissues were determined by quantitative Real-Time PCR, confirmed by immunohistochemistry (CCL20) or by flow cytometry analysis (CCR6). Chemotaxis of splenocytes or purified CD19⁺ B cells to tumor cell-derived CCL20, and angiogenesis of different CD19⁺ B subtypes responding to tumor cell-deri

SUBMITTER: He H 

PROVIDER: S-EPMC5446480 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature