MS2 Lysis of Escherichia coli Depends on Host Chaperone DnaJ.

ABSTRACT: The L protein of the single-stranded RNA phage MS2 causes lysis of Escherichia coli without inducing bacteriolytic activity or inhibiting net peptidoglycan (PG) synthesis. To find host genes required for L-mediated lysis, spontaneous Ill (insensitivity to Llysis) mutants were selected as survivors of L expression and shown to have a missense change of the highly conserved proline (P330Q) in the C-terminal domain of DnaJ. In the dnaJ_P330Q mutant host, L-mediated lysis is completely blocked at 30°C without affecting the intracellular levels of L. At higher temperatures (37°C and 42°C), both lysis and L accumulation are delayed. The lysis block at 30°C in the dnaJ_P330Q mutant was recessive and could be suppressed by L</

SUBMITTER: Chamakura KR 

PROVIDER: S-EPMC5446614 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature