Novel DNA Binding and Regulatory Activities for σ⁵⁴ (RpoN) in Salmonella enterica Serovar Typhimurium 14028s.

ABSTRACT: The variable sigma (σ) subunit of the bacterial RNA polymerase (RNAP) holoenzyme, which is responsible for promoter specificity and open complex formation, plays a strategic role in the response to environmental changes. Salmonella enterica serovar Typhimurium utilizes the housekeeping σ⁷⁰ and five alternative sigma factors, including σ⁵⁴ The σ⁵⁴-RNAP differs from other σ-RNAP holoenzymes in that it forms a stable closed complex with the promoter and requires ATP hydrolysis by an activated cognate bacterial enhancer binding protein (bEBP) to transition to an open complex and initiate transcription. In S. Typhimurium, σ⁵⁴-dependent promoters normally respond to one of 13 different bEBPs, each of which is activated under a specific gr

SUBMITTER: Bono AC 

PROVIDER: S-EPMC5446619 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature