Examination of Enterococcus faecalis Toxin-Antitoxin System Toxin Fst Function Utilizing a Pheromone-Inducible Expression Vector with Tight Repression and Broad Dynamic Range.

ABSTRACT: Tools for regulated gene expression in Enterococcus faecalis are extremely limited. In this report, we describe the construction of an expression vector for E. faecalis, designated pCIE, utilizing the P_Q pheromone-responsive promoter of plasmid pCF10. We demonstrate that this promoter is tightly repressed, responds to nanogram quantities of the peptide pheromone, and has a large dynamic range. To demonstrate its utility, the promoter was used to control expression of the toxic peptides of two par family toxin-antitoxin (TA) loci present in E. faecalis, par_pAD1 of the pAD1 plasmid and par_EF0409 located on the E. faecalis chromosome. The results demonstrated differences in the modes of regulation of toxin expre

SUBMITTER: Weaver KE 

PROVIDER: S-EPMC5446624 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature