MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway.

ABSTRACT:

Background

Current evidence indicates that miR-608 is widely down-regulated in various malignant tumors including liver cancer, colon cancer, lung cancer and glioma, and acts as a tumor suppressor by inhibiting cell proliferation, invasion and migration or by promoting apoptosis. The specific biological function of miR-608 in bladder cancer is still unknown.

Methods

qRT-PCR and Chromogenic in Situ Hybridization (CISH) was conducted to assess the expression of miR-608 in paired BCa tissues and adjacent non-tumor bladder urothelial tissues. Bisulfite sequencing PCR was used for DNA methylation analysis. CCK-8, colony formation and flow cytometry assays were performed, and a xenograft model was studied. Immunohistochemistry staining was performed with peroxidase and DAB. The ta

SUBMITTER: Liang Z 

PROVIDER: S-EPMC5446711 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature