Differentiation of Bifidobacterium longum subspecies longum and infantis by quantitative PCR using functional gene targets.

ABSTRACT:

Background

Members of the genus Bifidobacterium are abundant in the feces of babies during the exclusively-milk-diet period of life. Bifidobacterium longum is reported to be a common member of the infant fecal microbiota. However, B. longum is composed of three subspecies, two of which are represented in the bowel microbiota (B. longum subsp. longum; B. longum subsp. infantis). B. longum subspecies are not differentiated in many studies, so that their prevalence and relative abundances are not accurately known. This may largely be due to difficulty in assigning subspecies identity using DNA sequences of 16S rRNA or tuf genes that are commonly used in bacterial taxonomy.

Methods

We developed a qPCR method t

SUBMITTER: Lawley B 

PROVIDER: S-EPMC5446769 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature