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Development and Optimization of a Reverse Transcription Hemi-Nested PCR Primer for the Detection of Potato Mop-Top Virus at Quarantine Inspection Sites in Korea.

ABSTRACT: Potato mop-top virus (PMTV) is classified as a plant quarantine virus in Korea. It is tested at import inspection sites. In this study, two sets of reverse transcription-hemi-nested polymerase chain reaction primers were developed to diagnose PMTV. In addition, modified- positive control plasmid was developed for the identification of false positive results in plant quarantine. The assay is expected to be useful for improving the detection and diagnosis of PMTV.

SUBMITTER: Lee S 

PROVIDER: S-EPMC5446823 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Development and Optimization of a Reverse Transcription Hemi-Nested PCR Primer for the Detection of <i>Potato Mop</i>-<i>Top Virus</i> at Quarantine Inspection Sites in Korea.

Lee S S   Lee J Y JY   Kim H H   Kim J H JH   Rho J Y JY  

Indian journal of microbiology 20160927 2

<i>Potato mop</i>-<i>top virus</i> (PMTV) is classified as a plant quarantine virus in Korea. It is tested at import inspection sites. In this study, two sets of reverse transcription-hemi-nested polymerase chain reaction primers were developed to diagnose PMTV. In addition, modified- positive control plasmid was developed for the identification of false positive results in plant quarantine. The assay is expected to be useful for improving the detection and diagnosis of PMTV. ...[more]

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