Dataset Information

Deep Sequencing Reveals Highly Variable Gut Microbial Composition of Invasive Fish Mossambicus Tilapia (Oreochromis mossambicus) Collected from Two Different Habitats.

ABSTRACT: Tilapia (Oreochromis mossambicus) is one of the most invasive fish found throughout the World and emerged as a major threat to the indigenous fishes in many countries. Investigating the gut microbial diversity of such fishes is one of the ways to understand its physiology. In the present study, we have explored the gut microbial community structure of tilapia using 16S rRNA gene sequencing on the Illumina Miseq platform. Our study showed significant differences in tilapia gut microbiota collected from different habitats (i.e. river and lakes) suggesting the influence of habitat on the gut microbial diversity of tilapia. This study gives a first insight into the mossambicus tilapia gut microbiota and provides a reference for future studies.

SUBMITTER: Gaikwad SS

PROVIDER: S-EPMC5446831 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Deep Sequencing Reveals Highly Variable Gut Microbial Composition of Invasive Fish Mossambicus Tilapia (Oreochromis mossambicus) Collected from Two Different Habitats.

Gaikwad Swapnil Sopan SS Shouche Yogesh S YS Gade Wasudeo N WN

Indian journal of microbiology 20170207 2

Tilapia (Oreochromis mossambicus) is one of the most invasive fish found throughout the World and emerged as a major threat to the indigenous fishes in many countries. Investigating the gut microbial diversity of such fishes is one of the ways to understand its physiology. In the present study, we have explored the gut microbial community structure of tilapia using 16S rRNA gene sequencing on the Illumina Miseq platform. Our study showed significant differences in tilapia gu ...[more]

PMID: 28611502

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Project description:BackgroundTilapia is the common name for a group of cichlid fishes and is one of the most important aquacultured freshwater food fish. Mozambique tilapia and its hybrids, including red tilapia are main representatives of salt tolerant tilapias. A linkage map is an essential framework for mapping QTL for important traits, positional cloning of genes and understanding of genome evolution.ResultsWe constructed a consensus linkage map of Mozambique tilapia and red tilapia using 95 individuals from two F1 families and 401 microsatellites including 282 EST-derived markers. In addition, we conducted comparative mapping and searched for sex-determining loci on the whole genome. These 401 microsatellites were assigned to 22 linkage groups. The map spanned 1067.6 cM with an average inter-marker distance of 3.3 cM. Comparative mapping between tilapia and stickleback, medaka, pufferfish and zebrafish revealed clear homologous relationships between chromosomes from different species. We found evidence for the fusion of two sets of two independent chromosomes forming two new chromosome pairs, leading to a reduction of 24 chromosome pairs in their ancestor to 22 pairs in tilapias. The XY sex determination locus in Mozambique tilapia was mapped on LG1, and verified in five families containing 549 individuals. The major XY sex determination locus in red tilapia was located on LG22, and verified in two families containing 275 individuals.ConclusionsA first-generation linkage map of salt tolerant tilapia was constructed using 401 microsatellites. Two separate fusions of two sets of two independent chromosomes may lead to a reduction of 24 chromosome pairs in their ancestor to 22 pairs in tilapias. The XY sex-determining loci from Mozambique tilapia and red tilapia were mapped on LG1 and LG22, respectively. This map provides a useful resource for QTL mapping for important traits and comparative genome studies. The DNA markers linked to the sex-determining loci could be used in the selection of YY males for breeding all-male populations of salt tolerant tilapia, as well as in studies on mechanisms of sex determination in fish.

Dataset Information

Deep Sequencing Reveals Highly Variable Gut Microbial Composition of Invasive Fish Mossambicus Tilapia (Oreochromis mossambicus) Collected from Two Different Habitats.

Publications

Deep Sequencing Reveals Highly Variable Gut Microbial Composition of Invasive Fish Mossambicus Tilapia (<i>Oreochromis mossambicus</i>) Collected from Two Different Habitats.

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