The Hybrid Strategy of Thermoactinospora rubra YIM 77501^T for Utilizing Cellulose as a Carbon Source at Different Temperatures.

ABSTRACT: Thermoactinospora rubra YIM 77501^T is an aerobic, Gram-positive, spore-forming and cellulose degrading thermophilic actinomycete isolated from a sandy soil sample of a volcano. Its growth temperature range is 28-60°C. The genomic sequence of this strain revealed that there are 27 cellulase genes belonging to six glycoside hydrolase families. To understand the strategy that this strain uses to utilize carbon sources such as cellulose at different temperatures, comparative transcriptomics analysis of T. rubra YIM 77501^T was performed by growing it with cellulose (CMC) and without cellulose (replaced with glucose) at 30, 40, and 50°C, respectively. Transcriptomic analyses showed four cellulase genes (TrBG2, TrBG3, TrBG4, and ThrCel6B) were up-

SUBMITTER: Yin YR 

PROVIDER: S-EPMC5447088 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature