Probing fast ribozyme reactions under biological conditions with rapid quench-flow kinetics.

ABSTRACT: Reaction kinetics on the millisecond timescale pervade the protein and RNA fields. To study such reactions, investigators often perturb the system with abiological solution conditions or substrates in order to slow the rate to timescales accessible by hand mixing; however, such perturbations can change the rate-limiting step and obscure key folding and chemical steps that are found under biological conditions. Mechanical methods for collecting data on the millisecond timescale, which allow these perturbations to be avoided, have been developed over the last few decades. These methods are relatively simple and can be conducted on affordable and commercially available instruments. Here, we focus on using the rapid quench-flow technique to study the fast reaction kinetics of RNA enzymes, or r

SUBMITTER: Bingaman JL 

PROVIDER: S-EPMC5447473 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature