Cytidine deaminase Apobec3a induction in fallopian epithelium after exposure to follicular fluid.

ABSTRACT:

Objective

Ovarian carcinomas that originate from fallopian epithelial cells are suggested to arise due to repeated exposure to ovulatory follicular fluid (FF). Mechanistic explanation(s) for how this occurs are unknown. Here, we sought to understand if FF exposure to fallopian epithelial cells could induce DNA damage and expression of a known family of DNA mutators, apolipoprotein B mRNA editing enzyme, catalytic polypeptide (APOBEC) cytidine deaminases.

Methods

Follicular fluid and matched patient plasma samples were obtained from donors. Fallopian epithelial cells (FT33-TAg, FT189, FT190, and FT194) were cultured with FF or plasma for 24h, and cell proliferation and DNA damage were assessed. Effects of FF on Apobec gene expression were determined by qRT-PCR and western blo

SUBMITTER: Brachova P 

PROVIDER: S-EPMC5447478 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature