Copper Resistance in Aspergillus nidulans Relies on the P_I-Type ATPase CrpA, Regulated by the Transcription Factor AceA.

ABSTRACT: Copper homeostasis has been extensively studied in mammals, bacteria, and yeast, but it has not been well-documented in filamentous fungi. In this report, we investigated the basis of copper tolerance in the model fungus Aspergillus nidulans. Three genes involved in copper homeostasis have been characterized. First, crpA the A. nidulans ortholog of Candida albicans CaCRP1 gene encoding a P_I-type ATPase was identified. The phenotype of crpA deletion led to a severe sensitivity to Cu⁺² toxicity and a characteristic morphological growth defect in the presence of high copper concentration. CrpA displayed some promiscuity regarding metal species response. The expression pattern of crpA showed an initial strong elevation of mRNA and

SUBMITTER: Antsotegi-Uskola M 

PROVIDER: S-EPMC5447758 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature