Urate hydroperoxide oxidizes human peroxiredoxin 1 and peroxiredoxin 2.

ABSTRACT: Urate hydroperoxide is a product of the oxidation of uric acid by inflammatory heme peroxidases. The formation of urate hydroperoxide might be a key event in vascular inflammation, where there is large amount of uric acid and inflammatory peroxidases. Urate hydroperoxide oxidizes glutathione and sulfur-containing amino acids and is expected to react fast toward reactive thiols from peroxiredoxins (Prxs). The kinetics for the oxidation of the cytosolic 2-Cys Prx1 and Prx2 revealed that urate hydroperoxide oxidizes these enzymes at rates comparable with hydrogen peroxide. The second-order rate constants of these reactions were 4.9 × 10⁵ and 2.3 × 10⁶ m^-1 s^-1 for Prx1 and Prx2, respectively. Kinetic and simulation data suggest that the oxidation of

SUBMITTER: Carvalho LAC 

PROVIDER: S-EPMC5448098 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature