Scavenger receptor B1 (SR-B1) profoundly excludes high density lipoprotein (HDL) apolipoprotein AII as it nibbles HDL-cholesteryl ester.

ABSTRACT: Reverse cholesterol transport (transfer of macrophage-cholesterol in the subendothelial space of the arterial wall to the liver) is terminated by selective high density lipoprotein (HDL)-cholesteryl ester (CE) uptake, mediated by scavenger receptor class B, type 1 (SR-B1). We tested the validity of two models for this process: "gobbling," i.e. one-step transfer of all HDL-CE to the cell and "nibbling," multiple successive cycles of SR-B1-HDL association during which a few CEs transfer to the cell. Concurrently, we compared cellular uptake of apoAI with that of apoAII, which is more lipophilic than apoAI, using HDL-[³H]CE labeled with [¹²⁵I]apoAI or [¹²⁵I]apoAII. The studies were conducted in CHO-K1 and CHO-ldlA7 cells (LDLR^-/-) with (CHO-S

SUBMITTER: Gillard BK 

PROVIDER: S-EPMC5448121 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature