PI(3,4)P₂ plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells.

ABSTRACT: Phosphoinositides play pivotal roles in the regulation of cancer cell phenotypes. Among them, phosphatidylinositol 3,4-bisphosphate (PI(3,4)P₂ ) localizes to the invadopodia, and positively regulates tumor cell invasion. In this study, we examined the effect of PI(3,4)P₂ on focal adhesion dynamics in MDA-MB-231 basal breast cancer cells. Knockdown of SHIP2, a phosphatidylinositol 3,4,5-trisphosphatase (PIP₃ ) 5-phosphatase that generates PI(3,4)P₂ , in MDA-MB-231 breast cancer cells, induced the development of focal adhesions and cell spreading, leading to the suppression of invasion. In contrast, knockdown of PTEN, a 3-phosphatase that de-phosphorylates PIP₃ and PI(3,4)P₂ , induced cell shrinkage and increased cell invasion

SUBMITTER: Fukumoto M 

PROVIDER: S-EPMC5448597 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature