Cellulose Isolation Methodology for NMR Analysis of Cellulose Ultrastructure.

ABSTRACT: In order to obtain accurate information about the ultrastructure of cellulose from native biomass by ¹³C cross polarization magic angle spinning (CP/MAS) NMR spectroscopy the cellulose component must be isolated due to overlapping resonances from both lignin and hemicellulose. Typically, cellulose isolation has been achieved via holocellulose pulping to remove lignin followed by an acid hydrolysis procedure to remove the hemicellulose components. Using ¹³C CP/MAS NMR and non-linear line-fitting of the cellulose C₄ region, it was observed that the standard acid hydrolysis procedure caused an apparent increase in crystallinity of ~10% or less on the cellulose isolated from Populus holocellulose. We have examined the effect of the cellulose isolation method, particularly

SUBMITTER: Foston MB 

PROVIDER: S-EPMC5448851 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature