Characterization of Thiomonas delicata arsenite oxidase expressed in Escherichia coli.

ABSTRACT: Microbial arsenite oxidation is an essential biogeochemical process whereby more toxic arsenite is oxidized to the less toxic arsenate. Thiomonas strains represent an important arsenite oxidizer found ubiquitous in acid mine drainage. In the present study, the arsenite oxidase gene (aioBA) was cloned from Thiomonas delicata DSM 16361, expressed heterologously in E. coli and purified to homogeneity. The purified recombinant Aio consisted of two subunits with the respective molecular weights of 91 and 21 kDa according to SDS-PAGE. Aio catalysis was optimum at pH 5.5 and 50-55 °C. Aio exhibited stability under acidic conditions (pH 2.5-6). The V _max and K _m values of the enzyme were found to be 4 µmol min^-1 mg^-1 and 14.2 µM, respectively. SDS and Tr

SUBMITTER: Teoh WK 

PROVIDER: S-EPMC5449285 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature