Melting temperature highlights functionally important RNA structure and sequence elements in yeast mRNA coding regions.

ABSTRACT: Secondary structure elements in the coding regions of mRNAs play an important role in gene expression and regulation, but distinguishing functional from non-functional structures remains challenging. Here we investigate the dependence of sequence-structure relationships in the coding regions on temperature based on the recent PARTE data by Wan et al. Our main finding is that the regions with high and low thermostability (high Tm and low Tm regions) are under evolutionary pressure to preserve RNA secondary structure and primary sequence, respectively. Sequences of low Tm regions display a higher degree of evolutionary conservation compared to high Tm regions. Low Tm regions are under strong synonymous constraint, while high Tm regions are not. These findings imply that high Tm regions conta

SUBMITTER: Qi F 

PROVIDER: S-EPMC5449622 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature