Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China.

ABSTRACT:

Background

Alveolar echinococcosis (AE) is a life-threatening human disease caused by Echinococcus multilocularis transmitted between rodents and dogs/foxes in the Northern Hemisphere. The study aims to identify the genetic variation of the parasite in AE patients from China.

Methods

E. multilocularis isolates were collected from wild small mammals (n = 6) and AE patients (n = 56) from western China. Genomic DNA was extracted from different tissue samples including paraffin tissue blocks, ethanol fixed tissues and frozen tissues surgically removed. Two mitochondrial gene fragments (526 bp for cob and 474 bp for nad2) of E. multilocularis were amplified and sequenced.

Results

The parasite fragment sequences of cob fragments from AE patients showed two haplotypes, and n

SUBMITTER: Wu C 

PROVIDER: S-EPMC5450100 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature