FGF2/FGFR1 regulates autophagy in FGFR1-amplified non-small cell lung cancer cells.

ABSTRACT:

Background

Autophagy is a conserved catabolic process to degrade cellular organelles. The role of autophagy in cancer development is complex. Amplification of fibroblast growth factor receptor 1 (FGFR1) is one of the most frequent targets in lung squamous cell carcinoma (SQCC). Whether fibroblast growth factor 2 (FGF2)/FGFR1 contributes to the regulation of autophagy remains elusive.

Methods

Autophagic activity was evaluated by immunoblotting for microtubule-associated protein 1 light chain 3 (LC3), formation of GFP-LC3 puncta, and monodansylcadaverine (MDC) staining. The effect of autophagy inhibition on cell survival was assessed by cell viability and apoptosis assays.

Results

We elucidated that FGFR1 activation suppressed autophagy. Pharmacological or genetic inhib

SUBMITTER: Yuan H 

PROVIDER: S-EPMC5450166 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature