NLRP3 inflammasome activation in murine macrophages caused by Neospora caninum infection.

ABSTRACT:

Background

Neospora caninum is an intracellular parasite that causes significant economic losses in cattle industry. Understanding the host resistance mechanisms in the innate immune response to neosporosis could facilitate the exploration of approaches for controlling N. caninum infection. The NLR inflammasome is a multiprotein platform in the cell cytoplasm and plays critical roles in the host response against microbes.

Methods

Neospora caninum-infected wild-type (WT) macrophages and Nlrp3 ^-/- macrophages, and inhibitory approaches were used to investigate inflammasome activation and its role in N. caninum infection. Inflammasome RT Profiler PCR Arrays were used to identify the primary genes involved in N. caninum infection. The expression of the sensor protein

SUBMITTER: Wang X 

PROVIDER: S-EPMC5450200 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature