Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica.

ABSTRACT: Sponges, despite their simple body plan, discriminate between self and nonself with remarkable specificity. Sponge grafting experiments simulate the effects of natural self or nonself contact under laboratory conditions. Here we take a transcriptomic approach to investigate the temporal response to self and nonself grafts in the marine demosponge Amphimedon queenslandica. Auto- and allografts were established, observed and sampled over a period of three days, over which time the grafts either rejected or accepted, depending on the identity of the paired individuals, in a replicable and predictable manner. Fourteen transcriptomes were generated that spanned the auto- and allograft responses. Self grafts fuse completely in under three days, and the process appears to be controlled by

SUBMITTER: Grice LF 

PROVIDER: S-EPMC5450542 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature