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Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA.

ABSTRACT: Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.

SUBMITTER: Tarn BE 

PROVIDER: S-EPMC5450655 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA.

Tarn B E BE   Sung K-J KJ   Sikes H D HD  

Molecular systems design & engineering 20160907 3

Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides. ...[more]

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